5OQF
Crystal structure of a single chain trimer composed of the MHC 1 heavy chain H2-Kb WT, beta-2microglobulin, and ovalbumin derived peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.650, 90.080, 89.330 |
| Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
| Resolution | 83.268 - 2.270 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qri |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.939 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 83.268 |
| High resolution limit [Å] | 2.270 |
| Number of reflections | 44945 |
| <I/σ(I)> | 9.8 |
| Completeness [%] | 98.6 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.2M Am sulphate, 25% PEG 8K, 0.1M Na cacodylate pH 6 |
