5OOQ
Structure of the Mtr4 Nop53 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.231, 151.370, 133.043 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 66.500 - 3.200 |
| Rwork | 0.211 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xgj |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.500 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.117 | |
| Rpim | 0.005 | 0.908 |
| Number of reflections | 55220 | 4762 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.3 | 94.54 |
| Redundancy | 6.8 | |
| CC(1/2) | 0.316 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285.15 | 20% PEG 6000 200 mM Lithium citrate |
