5ONA

Drosophila Bag-of-marbles CBM peptide bound to human CAF40-CNOT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	106.600, 106.600, 263.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.408 - 2.700
R-factor	0.2102
Rwork	0.209
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cru
RMSD bond length	0.002
RMSD bond angle	0.437
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	48613	3529
<I/σ(I)>	15.5	1.2
Completeness [%]	99.9	99.5
Redundancy	11	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	295	1.5 M ammonium sulfate 20 mM MES (pH 6.0) 80 mM MES (pH 6.5)