5OM0
CH2 chimera of human 14-3-3 sigma with the Gli1 phosphopeptide around Ser640
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 110.426, 110.426, 174.114 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.000 - 3.200 |
| R-factor | 0.22 |
| Rwork | 0.215 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lu1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 3.380 |
| High resolution limit [Å] | 3.200 | 3.190 |
| Rmerge | 0.230 | 4.300 |
| Number of reflections | 10910 | |
| <I/σ(I)> | 14.3 | 0.91 |
| Completeness [%] | 99.6 | 98.2 |
| Redundancy | 23 | 22.3 |
| CC(1/2) | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M bis-Tris (pH 6.5), 2 M ammonium sulfate |
