5OLR
Rhamnogalacturonan lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.458, 123.872, 137.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.400 - 1.070 |
| R-factor | 0.1458 |
| Rwork | 0.145 |
| R-free | 0.16643 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.538 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.090 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Number of reflections | 536956 | 9262 |
| <I/σ(I)> | 11.3 | 0.9 |
| Completeness [%] | 94.3 | 33.1 |
| Redundancy | 3.2 | 1.7 |
| CC(1/2) | 0.998 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM succinic acid, sodium phosphate glycine buffer pH 6.0 and 25 % (w/v) PEG 1500 |
