5OLJ
Crystal structure of Porphyromonas gingivalis dipeptidyl peptidase 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | Xenocs GeniX 3D Cu HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-07-09 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 109.100, 109.100, 157.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.870 - 2.200 |
R-factor | 0.22502 |
Rwork | 0.224 |
R-free | 0.25451 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kby |
RMSD bond length | 0.014 |
RMSD bond angle | 1.634 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 48811 |
<I/σ(I)> | 10.8 |
Completeness [%] | 99.7 |
Redundancy | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290 | 10% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 0.02 M sodium L-glutamate, 0.02 M DL-alanine, 0.02 M glycine, 0.02 M DL-lysine, 0.02 M DLserine, and 0.1 M BICINE/TRIS pH 8.5 |