5OKM
Crystal structure of human SHIP2 Phosphatase-C2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 136.037, 175.836, 176.893 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.960 - 1.960 |
R-factor | 0.18091 |
Rwork | 0.179 |
R-free | 0.20750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nr8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.306 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.970 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Rpim | 0.033 | 0.368 |
Number of reflections | 287002 | |
<I/σ(I)> | 14.8 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 | 6.7 |
CC(1/2) | 0.999 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M BisTris propane pH 7, 0.2 M NaNO3, 20% PEG 3350, 0.025% CH2Cl2, 2 mM TCEP |