5OKI

Crystal structure of the Ctf18-1-8 module from Ctf18-RFC in complex with a 63 kDa fragment of DNA Polymerase epsilon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	C 2 2 21
Unit cell lengths	121.678, 145.826, 378.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.724 - 4.500
R-factor	0.2935
Rwork	0.293
R-free	0.31210
RMSD bond length	0.004
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.720	47.720	4.930
High resolution limit [Å]	4.500	11.020	4.500
Rmerge	0.668	0.075	2.548
Rmeas	0.725	0.082	2.765
Rpim	0.278	0.034	1.067
Total number of observations	135607	8239	31990
Number of reflections	20450	1492	4814
<I/σ(I)>	3.5	18.9	0.8
Completeness [%]	99.9	98.9	100
Redundancy	6.6	5.5	6.6
CC(1/2)	0.969	0.997	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	12% PEG 10000, 0.1 M MES pH 6.5, 0.2 M ammonium chloride