5OKI
Crystal structure of the Ctf18-1-8 module from Ctf18-RFC in complex with a 63 kDa fragment of DNA Polymerase epsilon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 121.678, 145.826, 378.752 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.724 - 4.500 |
R-factor | 0.2935 |
Rwork | 0.293 |
R-free | 0.31210 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.043 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.720 | 47.720 | 4.930 |
High resolution limit [Å] | 4.500 | 11.020 | 4.500 |
Rmerge | 0.668 | 0.075 | 2.548 |
Rmeas | 0.725 | 0.082 | 2.765 |
Rpim | 0.278 | 0.034 | 1.067 |
Total number of observations | 135607 | 8239 | 31990 |
Number of reflections | 20450 | 1492 | 4814 |
<I/σ(I)> | 3.5 | 18.9 | 0.8 |
Completeness [%] | 99.9 | 98.9 | 100 |
Redundancy | 6.6 | 5.5 | 6.6 |
CC(1/2) | 0.969 | 0.997 | 0.323 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 12% PEG 10000, 0.1 M MES pH 6.5, 0.2 M ammonium chloride |