5OKB
High resolution structure of native Gan1D, a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus
Replaces: 4ZEHExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 68.967, 78.099, 92.089 |
Unit cell angles | 102.36, 93.54, 91.74 |
Refinement procedure
Resolution | 30.829 - 1.331 |
R-factor | 0.1222 |
Rwork | 0.122 |
R-free | 0.14540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ZEH |
RMSD bond length | 0.006 |
RMSD bond angle | 0.917 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.350 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmerge | 0.055 | 0.596 |
Number of reflections | 429175 | |
<I/σ(I)> | 11.9 | 2.4 |
Completeness [%] | 95.5 | 92.3 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16-19% PEG 8K, 3% MPD, 0.1M imidazole buffer pH 6.5 |