5OKB
High resolution structure of native Gan1D, a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus
Replaces: 4ZEHExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.967, 78.099, 92.089 |
| Unit cell angles | 102.36, 93.54, 91.74 |
Refinement procedure
| Resolution | 30.829 - 1.331 |
| R-factor | 0.1222 |
| Rwork | 0.122 |
| R-free | 0.14540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ZEH |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.917 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.350 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.055 | 0.596 |
| Number of reflections | 429175 | |
| <I/σ(I)> | 11.9 | 2.4 |
| Completeness [%] | 95.5 | 92.3 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16-19% PEG 8K, 3% MPD, 0.1M imidazole buffer pH 6.5 |
