5OI7
Human CEP85 - coiled coil domain 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-10-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 37.930, 73.880, 128.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.976 - 1.670 |
| R-factor | 0.2043 |
| Rwork | 0.203 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q6q |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.538 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.980 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.202 | 1.552 |
| Rpim | 0.063 | 0.506 |
| Number of reflections | 21341 | 2852 |
| <I/σ(I)> | 8.6 | 1.4 |
| Completeness [%] | 98.9 | 92.2 |
| Redundancy | 11.1 | 10.1 |
| CC(1/2) | 0.474 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 100 mM Na-HEPES, pH 7.7 200 mM Na-Citrate 36 % (v/v) MPD |
