5OGK
Crystal structure of a nucleotide sugar transporter with bound nucleotide sugar.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 83 |
Detector technology | PIXEL |
Collection date | 2017-05-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 |
Unit cell lengths | 45.319, 101.694, 180.192 |
Unit cell angles | 89.86, 90.07, 90.11 |
Refinement procedure
Resolution | 44.328 - 3.600 |
R-factor | 0.2597 |
Rwork | 0.257 |
R-free | 0.31270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5oge |
RMSD bond length | 0.003 |
RMSD bond angle | 0.652 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.300 | 3.760 |
High resolution limit [Å] | 3.600 | 3.600 |
Rpim | 0.200 | 1.110 |
Number of reflections | 36110 | 4518 |
<I/σ(I)> | 3.8 | 0.6 |
Completeness [%] | 96.8 | 97.2 |
Redundancy | 1.8 | 1.8 |
CC(1/2) | 0.980 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5 | 277 | 26 - 30 % (v/v) PEG 400, 0.1 M sodium citrate pH 5.0 and 75 mM sodium chloride or sodium acetate. GDP-mannose was soaked in at 20mM overnight. |