5OGE
Crystal structure of a nucleotide sugar transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-10-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 2.70 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.570, 102.910, 181.410 |
| Unit cell angles | 89.92, 90.12, 90.06 |
Refinement procedure
| Resolution | 45.350 - 3.220 |
| R-factor | 0.281 |
| Rwork | 0.280 |
| R-free | 0.30600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.350 | 3.300 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.162 | 0.688 |
| Rpim | 0.070 | 0.688 |
| Number of reflections | 48151 | 2345 |
| <I/σ(I)> | 8.9 | 1.1 |
| Completeness [%] | 90.3 | 60.1 |
| Redundancy | 8.4 | 2.9 |
| CC(1/2) | 0.990 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 277 | 26 - 30 % (v/v) PEG 400, 0.1 M sodium citrate pH 5.0 and 75 mM sodium chloride or sodium acetate |
