5OGB
Human Cellular Retinoic Acid Binding Protein II (CRABPII) with bound synthetic retinoid DC360.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0388 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.050, 45.580, 36.670 |
| Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
| Resolution | 35.770 - 1.800 |
| R-factor | 0.17675 |
| Rwork | 0.173 |
| R-free | 0.23582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fr3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.681 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.770 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 9030 |
| <I/σ(I)> | 6.2 |
| Completeness [%] | 91.1 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 4000, Na Acetate |
