5OER
Hen egg-white lysozyme refined against 5000 9 keV diffraction patterns
Experimental procedure
| Experimental method | MAD |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-01-01 |
| Detector | MPCCD |
| Wavelength(s) | 1.38 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.100, 79.100, 39.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.930 - 1.900 |
| R-factor | 0.18698 |
| Rwork | 0.186 |
| R-free | 0.21435 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.659 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 10028 | |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 68 | 48 |
| CC(1/2) | 0.876 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | Sodium chloride |
