5OBM

Crystal structure of Gentamicin bound to the yeast 80S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	90
Detector technology	PIXEL
Collection date	2015-07-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	436.630, 287.000, 304.730
Unit cell angles	90.00, 99.08, 90.00

Refinement procedure

Resolution	143.721 - 3.400
R-factor	0.1896
Rwork	0.189
R-free	0.23790
RMSD bond length	0.008
RMSD bond angle	1.226
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	143.721	143.721	3.500
High resolution limit [Å]	3.400	105.000	3.400
Rmerge	0.297		1.554
Rmeas	0.308		1.751
Number of reflections	1013190		83925
<I/σ(I)>	9.21		0.98
Completeness [%]	99.9		99.6
Redundancy	15.171		4.683
CC(1/2)	0.995		0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine