5OBG

Crystal structure of glycine binding protein in complex with strychnine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	150
Detector technology	CCD
Collection date	2017-04-11
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54157
Spacegroup name	P 2 21 21
Unit cell lengths	90.353, 100.040, 163.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.920 - 2.000
R-factor	0.19928
Rwork	0.197
R-free	0.24961
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oan
RMSD bond length	0.018
RMSD bond angle	1.950
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.920	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.147	0.589
Number of reflections	100843	4919
<I/σ(I)>	6.1	1.9
Completeness [%]	100.0	100
Redundancy	6.2	6.2
CC(1/2)	0.992	0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Reservoir buffer: 20 % PEG 3350, 0.2 M MgOAc Protein buffer: 50 mM tris, 250 mM NaCl, pH 7.5, 0.5 mM strychnine