5OBB
Structure of a modified mouse H chain ferritin with a lanthanide binding motif in complex with Terbium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 237.251, 237.563, 237.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.480 - 2.650 |
| R-factor | 0.1679 |
| Rwork | 0.168 |
| R-free | 0.17350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wnw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.628 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 191449 | 8966 |
| <I/σ(I)> | 12.6 | 1.4 |
| Completeness [%] | 99.2 | 94.3 |
| Redundancy | 6.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | ammonium sulphate, TRIS-HCl |
