5O85
p34-p44 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28236 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 81.407, 120.388, 128.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.700 - 3.400 |
| R-factor | 0.233 |
| Rwork | 0.240 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pn7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 3.390 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.149 | 3.092 |
| Rpim | 0.061 | 1.272 |
| Number of reflections | 141682 | |
| <I/σ(I)> | 8.9 | 1 |
| Completeness [%] | 99.4 | 96.4 |
| Redundancy | 13.1 | 12.8 |
| CC(1/2) | 0.995 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 mM Tris-HCl or Hepes (pH 7.0 to 8.5) and 20-40% PEG 4000 |
