5O6Q
High pressure flash cooled hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.950, 77.950, 37.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.975 - 1.450 |
| R-factor | 0.1748 |
| Rwork | 0.172 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vb1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.752 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.980 | 1.501 |
| High resolution limit [Å] | 1.450 | 1.449 |
| Rmerge | 0.048 | 1.856 |
| Rmeas | 0.049 | 1.897 |
| Number of reflections | 20967 | |
| <I/σ(I)> | 34.46 | |
| Completeness [%] | 99.9 | |
| Redundancy | 24.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | lysozyme was dissolved in 0.02 M sodium acetate pH 4.6 to yield 20 mg/ml and mixed 1:1 with 0.8 M sodium chloride and 0.1 M sodium acetate pH 4.6 |
