5O5H
Crystal structure of the human BRPF1 bromodomain in complex with BZ053
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.027, 61.027, 63.787 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.697 - 1.850 |
R-factor | 0.1944 |
Rwork | 0.190 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lc2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.098 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.700 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.041 | 0.640 |
Rpim | 0.014 | 0.212 |
Number of reflections | 12134 | 740 |
<I/σ(I)> | 43.9 | 5.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.6 | 19.2 |
CC(1/2) | 1.000 | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-Tris Propane, pH6.5, 0.2 M Sodium Nitrate, 20% PEG3350 |