5O5E

Crystal structure of human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) (V264G mutant) in complex with tunicamycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 65 2 2
Unit cell lengths	102.091, 102.091, 240.061
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.870 - 3.400
R-factor	0.2298
Rwork	0.229
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	Aimless (0.5.25)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.330	3.490
High resolution limit [Å]	3.400	3.400
Rmerge	0.089	2.295
Rpim	0.032	1.044
Number of reflections	10832	761
<I/σ(I)>	13.4	1.2
Completeness [%]	100.0	99.8
Redundancy	9.3	9.8
CC(1/2)	0.999	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1M bicine pH 8.5 -- 0.05M sodium chloride -- 36% PEG200