5O4Y
Structure of human PD-L1 in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.550, 80.950, 54.230 |
| Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
| Resolution | 29.780 - 2.300 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c3t |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.780 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.107 | 0.552 |
| Number of reflections | 18522 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium acetate (pH 5.5), 0.1 M Bis-Tris, 25% PEG 3350 |
