5O4Q
Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus with 0.25 mM copper sulfate on growth medium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.841, 94.850, 116.614 |
| Unit cell angles | 90.04, 90.29, 91.49 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18846 |
| Rwork | 0.187 |
| R-free | 0.21309 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o3k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.119 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 235821 |
| <I/σ(I)> | 6.8 |
| Completeness [%] | 95.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 23% PEG 4000, 0.05M Tris-HCl |
