5O4O
HER3 in complex with Fab MF3178
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-24 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96771 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 64.380, 144.020, 218.756 |
| Unit cell angles | 90.00, 97.91, 90.00 |
Refinement procedure
| Resolution | 59.971 - 3.400 |
| R-factor | 0.2171 |
| Rwork | 0.215 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6b 3skj 4hcr |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.514 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.970 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rpim | 0.134 | 0.372 |
| Number of reflections | 27028 | 4355 |
| <I/σ(I)> | 17.7 | 2.5 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.942 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M bis tris propane pH 8.5, 0.2 M potassium thyocianate and 20% w/v PEG 3350 |
