5O49

Human FGF in complex with a covalent inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	C 1 2 1
Unit cell lengths	209.550, 57.590, 65.620
Unit cell angles	90.00, 107.64, 90.00

Refinement procedure

Resolution	49.920 - 1.910
R-factor	0.2
R-free	0.22000
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.920	1.960
High resolution limit [Å]	1.910	1.910
Rmerge	0.036	0.735
Number of reflections	57050	4147
<I/σ(I)>	13.9	1.5
Completeness [%]	98.2	97.8
Redundancy	3.2	2.8
CC(1/2)	0.998	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	18-20% (w/v) PEG8000, 200mM ammonium sulphate, 100mM PCTP pH 6.75 and 20% (v/v) ethylene glycol