5O3F

Human Brd2(BD2) mutant in complex with ET-Am1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	51.965, 71.548, 31.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.046 - 1.750
R-factor	0.1746
Rwork	0.173
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qeu
RMSD bond length	0.006
RMSD bond angle	1.015
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.050	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.112	0.144
Number of reflections	12439	269
<I/σ(I)>	7.72
Completeness [%]	99.7	99.92
Redundancy	3.9	4
CC(1/2)	0.992	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Tris pH 8.5 60% Pentaerythritol propoxylate (5/4 PO/OH)