5O3A

Human Brd2(BD2) mutant in complex with ET

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-19
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	I 41 2 2
Unit cell lengths	126.799, 126.799, 43.194
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.097 - 2.400
R-factor	0.208
Rwork	0.205
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qeu
RMSD bond length	0.011
RMSD bond angle	1.192
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.100	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.331
Number of reflections	7114	696
<I/σ(I)>	17.11	5.14
Completeness [%]	99.2	100
Redundancy	15.3	14.6
CC(1/2)	0.999	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.25	293	0.1M Tris pH 8.25 55% Pentaerythritol propoxylate (5/4 PO/OH) 0.2M Imidazole