5O32
The structure of complement complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-04 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.988 |
Spacegroup name | P 1 |
Unit cell lengths | 116.370, 122.790, 157.200 |
Unit cell angles | 69.76, 76.10, 70.13 |
Refinement procedure
Resolution | 49.134 - 4.206 |
R-factor | 0.230193326461 |
Rwork | 0.229 |
R-free | 0.25407 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.838 |
Data reduction software | XDS (20161101) |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.200 |
High resolution limit [Å] | 4.200 |
Rmerge | 0.174 |
Rpim | 0.109 |
Number of reflections | 55430 |
<I/σ(I)> | 6.1 |
Completeness [%] | 93.4 |
Redundancy | 3.5 |
CC(1/2) | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 60mM sodium malonate PH5.0 6% w/v PEG3350 |