5O2Z
Domain swap dimer of the G167R variant of gelsolin second domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0109 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.290, 44.420, 58.010 |
| Unit cell angles | 90.00, 110.13, 90.00 |
Refinement procedure
| Resolution | 45.386 - 1.700 |
| R-factor | 0.2014 |
| Rwork | 0.199 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5faf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.767 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.460 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.124 | 0.802 |
| Number of reflections | 26967 | 7132 |
| <I/σ(I)> | 9.82 | 1.9 |
| Completeness [%] | 95.5 | 98.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M ammonium acetate, 0.1M sodium citrate, 30% PEG 4000 |
