5O28
E. coli FolD apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.590, 79.840, 101.410 |
| Unit cell angles | 90.00, 113.26, 90.00 |
Refinement procedure
| Resolution | 36.140 - 1.890 |
| R-factor | 0.1849 |
| Rwork | 0.184 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dia |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.637 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.140 | 1.940 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.084 | 0.790 |
| Number of reflections | 116659 | |
| <I/σ(I)> | 17.9 | 2.4 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium acetate, 0.1 M sodium cacodylate pH 6.5 and 30 % (w/v) PEG 8000 |
