5O26
Crystal structure of WNK3 kinase domain in a diphosphorylated state and in complex with AMP-PNP/Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 |
Unit cell lengths | 36.163, 56.400, 71.236 |
Unit cell angles | 79.79, 80.73, 90.00 |
Refinement procedure
Resolution | 47.657 - 2.379 |
R-factor | 0.231 |
Rwork | 0.228 |
R-free | 0.27810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.480 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.483 |
High resolution limit [Å] | 2.379 |
Number of reflections | 21510 |
<I/σ(I)> | 4.8 |
Completeness [%] | 98.0 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG3350, 0.1M bis-tris pH 5.5 |