5O23

Crystal structure of WNK3 kinase domain in a monophosphorylated apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 32 2 1
Unit cell lengths	124.020, 124.020, 107.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	75.930 - 2.250
R-factor	0.2068
Rwork	0.205
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.432
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	75.930
High resolution limit [Å]	2.250
Number of reflections	45616
<I/σ(I)>	24.8
Completeness [%]	99.9
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG Smear Medium, 0.2 M lithium sulfate, 0.1 M ADA pH 6.5