5O0X
Crystal structure of dehydrogenase domain of Cylindrospermum stagnale NADPH-Oxidase 5 (NOX5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 128.279, 128.279, 72.519 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 111.090 - 2.200 |
| R-factor | 0.18546 |
| Rwork | 0.184 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.779 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.103 | 1.237 |
| Number of reflections | 33160 | 3002 |
| <I/σ(I)> | 11.4 | 1.5 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 5.6 | 5.8 |
| CC(1/2) | 0.993 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293 | 60 mM Ca-acetate, 80 mM NaCacodylate pH 6.5, 12-16% PEG 8000, 20% glycerol |
