5O0R
Deglycosylated Nogo Receptor with native disulfide structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.728, 111.654, 114.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.105 - 2.500 |
| R-factor | 0.2546 |
| Rwork | 0.252 |
| R-free | 0.30260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ozn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.547 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 21264 | |
| <I/σ(I)> | 3.8 | 1.4 |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 3.4 | 3.7 |
| CC(1/2) | 0.981 | 0.663 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 1.8 M NaH2PO4/K2HPO4 pH 5.0 |
