5O0Q
Deglycosylated Nogo Receptor with native disulfide structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-17 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 132.121, 46.332, 132.232 |
| Unit cell angles | 90.00, 91.93, 90.00 |
Refinement procedure
| Resolution | 45.938 - 2.500 |
| R-factor | 0.2137 |
| Rwork | 0.211 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ozn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.569 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.940 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.181 | 1.091 |
| Number of reflections | 53288 | |
| <I/σ(I)> | 5.8 | 1 |
| Completeness [%] | 94.8 | 96.8 |
| Redundancy | 2.6 | 2.4 |
| CC(1/2) | 0.992 | 0.472 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.2 M NaH2PO4, 20% PEG3350 |
