5O0O

Deglycosylated Nogo Receptor with native disulfide structure 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99990
Spacegroup name	P 41 21 2
Unit cell lengths	168.490, 168.490, 256.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.386 - 2.200
R-factor	0.1687
Rwork	0.167
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ozn
RMSD bond length	0.004
RMSD bond angle	0.850
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.390	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	185934
<I/σ(I)>	11.4	2.2
Completeness [%]	100.0	100
Redundancy	8.5	8.7
CC(1/2)	0.998	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	2.5 M sodium chloride, 100 mM Sodium Acetate/Acetic acid pH 4.5, 200 mM Lithium Sulfate