5O0M
Deglycosylated Nogo Receptor with native disulfide structure 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99990 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.610, 112.070, 115.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.030 - 1.900 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.22240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ozn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.869 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.030 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 48250 | |
| <I/σ(I)> | 6 | 1.5 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 4.6 | 4.7 |
| CC(1/2) | 0.994 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.05 M MIB buffer pH 5.0, 12.5% PEG1500 |
