5O05

GII.10 Vietnam 026 norovirus protruding domain in complex with Nanobody Nano-42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-09
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8729
Spacegroup name	P 21 21 21
Unit cell lengths	87.930, 105.360, 107.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.327 - 2.000
R-factor	0.182
Rwork	0.180
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ONU and 4X4C
RMSD bond length	0.009
RMSD bond angle	1.082
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.327	47.327	2.120
High resolution limit [Å]	2.000	5.950	2.000
Rmerge	0.090	0.025	0.742
Rmeas	0.102	0.028	0.838
Number of reflections	68217	2795	10775
<I/σ(I)>	13.08	45.98	2.15
Completeness [%]	99.6	99.1	98.8
Redundancy	4.497	4.392	4.646
CC(1/2)	0.998	0.999	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M potassium iodide, 20% (w/v) PEG3350