5O05
GII.10 Vietnam 026 norovirus protruding domain in complex with Nanobody Nano-42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-09 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8729 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.930, 105.360, 107.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.327 - 2.000 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ONU and 4X4C |
RMSD bond length | 0.009 |
RMSD bond angle | 1.082 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.327 | 47.327 | 2.120 |
High resolution limit [Å] | 2.000 | 5.950 | 2.000 |
Rmerge | 0.090 | 0.025 | 0.742 |
Rmeas | 0.102 | 0.028 | 0.838 |
Number of reflections | 68217 | 2795 | 10775 |
<I/σ(I)> | 13.08 | 45.98 | 2.15 |
Completeness [%] | 99.6 | 99.1 | 98.8 |
Redundancy | 4.497 | 4.392 | 4.646 |
CC(1/2) | 0.998 | 0.999 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M potassium iodide, 20% (w/v) PEG3350 |