5O02

GII.17 Kawasaki323 protruding domain in complex with Nanobody Nano-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-07
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.965
Spacegroup name	C 1 2 1
Unit cell lengths	118.470, 64.040, 70.030
Unit cell angles	90.00, 97.26, 90.00

Refinement procedure

Resolution	45.060 - 1.720
R-factor	0.1518
Rwork	0.150
R-free	0.18050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5F4M and 4X4C
RMSD bond length	0.006
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.060	45.060	1.800
High resolution limit [Å]	1.690	5.050	1.690
Rmerge	0.068	0.032	0.427
Rmeas	0.082	0.038	0.521
Number of reflections	55894	2222	8412
<I/σ(I)>	11.55	31.51	2.26
Completeness [%]	96.5	97.8	90.2
Redundancy	3.165	3.378	2.93
CC(1/2)	0.997	0.997	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2 M calcium acetate, 10% (w/v) PEG8000, 0.1 M imidazole