5NZK
Crystal structure of UDP-glucose pyrophosphorylase from Leishmania major in complex with phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.655, 107.381, 151.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.081 - 2.450 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oef |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.450 | |
| Number of reflections | 22170 | |
| <I/σ(I)> | 17.27 | 2.28 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 24% PEG 3350, 100 mM Bis-Tris pH 6.8, 0.2 M Li2SO4, 2 mM DTT |
