5NZD
The structure of the thermobifida fusca guanidine III riboswitch in space group P212121.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92019 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.460, 64.613, 84.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.146 - 2.007 |
| R-factor | 0.2213 |
| Rwork | 0.220 |
| R-free | 0.24500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.587 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.146 | 2.040 |
| High resolution limit [Å] | 2.007 | 2.007 |
| Rmerge | 0.049 | 1.282 |
| Rpim | 0.025 | 0.623 |
| Number of reflections | 33860 | 882 |
| <I/σ(I)> | 11.17 | |
| Completeness [%] | 99.8 | 96.1 |
| Redundancy | 5.12 | 5.2 |
| CC(1/2) | 0.985 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M Magnesium acetate, 0.05 M MES pH 5.6, 20% v/v 2-Methyl-2,4-pentanediol |
