5NXQ

Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to a stapled Sld5 CIP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976300
Spacegroup name	P 2 21 21
Unit cell lengths	88.679, 100.287, 219.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.209 - 2.413
R-factor	0.1858
Rwork	0.184
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c8s
RMSD bond length	0.002
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.210	2.499
High resolution limit [Å]	2.413	2.413
Rmerge	0.142	2.453
Rpim	0.041	0.735
Number of reflections	76177	7499
<I/σ(I)>	14.8	1.03
Completeness [%]	100.0	99.83
Redundancy	13.2	12.1
CC(1/2)	0.999	0.329

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M tri-sodium citrate pH 6.2 7.5-9% (w/v) PEG 8000 0.40-0.65 M NaCI