5NXC
LIM Domain Kinase 1 (LIMK1) In Complex With PF-00477736
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.387, 95.836, 84.392 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.970 - 2.250 |
R-factor | 0.24188 |
Rwork | 0.239 |
R-free | 0.29837 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yi6 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.749 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.390 | 2.280 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.220 | 0.920 |
Number of reflections | 17463 | 857 |
<I/σ(I)> | 6.3 | 2.1 |
Completeness [%] | 100.0 | 99.7 |
Redundancy | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M HEPES pH 7.0 0.2 M MgCl2 10% PEG8K |