5NWL

Crystal structure of a human RAD51-ATP filament.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.95373
Spacegroup name	P 1 21 1
Unit cell lengths	117.700, 128.000, 230.100
Unit cell angles	90.00, 90.30, 90.00

Refinement procedure

Resolution	49.140 - 3.930
R-factor	0.2708
Rwork	0.268
R-free	0.31670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n0w
RMSD bond length	0.003
RMSD bond angle	0.429
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.140	4.020
High resolution limit [Å]	3.930	3.930
Rmerge	0.287	2.888
Rpim	0.168	1.683
Number of reflections	61077	4149
<I/σ(I)>	3.8	0.5
Completeness [%]	99.2	92.2
Redundancy	3.8	3.8
CC(1/2)	0.991	0.263

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 MES pH 5.2 MPD