5NVA

Substrate-bound outward-open state of a Na+-coupled sialic acid symporter reveals a novel Na+-site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	48.788, 97.764, 151.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.176 - 2.260
R-factor	0.2264
Rwork	0.224
R-free	0.26080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.506
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	82.180
High resolution limit [Å]	2.260
Number of reflections	33923
<I/σ(I)>	8.39
Completeness [%]	96.6
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293.15	0.1M sodium citrate pH5, 0.2-0.25M potassium chloride, 30-40% (w/v) pentaerythritol propoxylate (5/4/PO/OH)