5NUZ

Junin virus GP1 glycoprotein in complex with an antibody Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	97.090, 101.910, 147.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.930 - 1.850
R-factor	0.1858
Rwork	0.184
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.338
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.930	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.131	1.780
Number of reflections	124898	9101
<I/σ(I)>	15.6	1.7
Completeness [%]	99.7	99.6
Redundancy	11.9	12
CC(1/2)	0.998	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	20 % (v/v) 2-propanol, 20 % (w/v) PEG 4000, 0.1 M tri-Sodium Citrate (pH 5.6)