5NTH
Structure of Leucyl aminopeptidase from Leishmania major in complex with actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97781 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 116.439, 116.439, 91.491 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.110 - 2.500 |
| R-factor | 0.19649 |
| Rwork | 0.194 |
| R-free | 0.24486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nsk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.642 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.110 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.150 | 0.747 |
| Number of reflections | 21850 | |
| <I/σ(I)> | 8.2 | 2 |
| Completeness [%] | 87.8 | 90.2 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.8 M Succinic acid pH 7.0, 3 % (w/v) 1,5-Diamino-pentane di-hydrochloride, 5 mM MnSO4, 5 mM actinonin |
