5NTC

Crystal structure of mouse CARM1 in complex with inhibitor SA0678

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.276
Spacegroup name	P 21 21 2
Unit cell lengths	74.909, 99.151, 206.873
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.941 - 2.250
R-factor	0.1799
Rwork	0.178
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.005
RMSD bond angle	1.090
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	73787
<I/σ(I)>	14.8	5.3
Completeness [%]	99.8	100
Redundancy	6.3	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	BTP pH 7.5 100 mM, PEG 2000 MME 25 %, NaCl 50 mM