5NT7

Structure of the LOTUS domain of Oskar in complex with the C-terminal RecA-like domain of Vasa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2014-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97908
Spacegroup name	P 1
Unit cell lengths	38.950, 39.040, 97.480
Unit cell angles	89.28, 91.85, 99.26

Refinement procedure

Resolution	48.713 - 1.400
R-factor	0.1696
Rwork	0.169
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a49 2db3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.048	1.382
Number of reflections	102457
<I/σ(I)>	10.44	0.74
Completeness [%]	91.4	87.5
Redundancy	2.16	2.18
CC(1/2)		0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM Tris pH 7.5, 200 mM potassium thiocyanate, 16% PEG 3350