5NQY

Structure of a fHbp(V1.4):PorA(P1.16) chimera. Fusion at fHbp position 309.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97623
Spacegroup name	P 21 21 21
Unit cell lengths	56.850, 62.640, 88.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.165 - 2.600
R-factor	0.2285
Rwork	0.226
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ayd
RMSD bond length	0.002
RMSD bond angle	0.449
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.170	2.670
High resolution limit [Å]	2.600	2.600
Rmerge	0.073	0.845
Rpim	0.032	0.356
Number of reflections	10208	743
<I/σ(I)>	16.7	2.3
Completeness [%]	99.6	99.4
Redundancy	6.4	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2M potassium formate, 20%(w/v) PEG3350